Doxorubicin is an effective antimitotic agent but whose use is limited by reported cases of reproductive toxicity attributed in part to oxidative stress. There is paucity of information on whether L-carnitine, a common and potent antioxidant mitigates doxorubicin-induced male reproductive impairment, hence this study. Fifteen (15) male Wistar rats used for the study were randomly divided into control, doxorubicin-only and doxorubian+L-carnitine groups of 5 rats each. Duration of daily administration with L-carnitine and weekly with doxorubicin was 28 days after which animals were sacrificed and relevant samples obtained for determination of parameters. Results show significant (p<0.05) decrease in relative testicular weight in the doxorubicin-only group compared with control, but significantly (p<0.001) higher in the Doxorubicin + L-carnitine groups compared with doxorubicin-only group. Sperm count, motility, and viability were significantly (p<0.05) reduced in the Doxorubicin-only compared with control group but significantly (p<0.05) higher in the doxorubicin + L-carnitine compared with Doxorubicin-only group. Teratozotospermia was significantly higher in Doxorubicin-only than in the control (p<0.05) but decreased in Doxorubicin+L-carnitine group (P<0.05) compared with doxorubicin-only group. Serum testosterone, luteinizing hormone and follicle-stimulating hormone concentrations were significantly (p<0.05) decreased in doxorubicin-only compared with control but significantly (p<0.05) higher in doxorubicin + L–carnitine compared with the doxorubicin-only groups. We therefore conclude that L-carnitine ameliorates doxorubicin-induced male reproductive toxicity in Wistar rats probably via its anti-oxidant potentials.